Coding

Part:BBa_K2022005:Design

Designed by: Daniel Winter   Group: iGEM16_UNSW_Australia   (2016-10-12)


alMGS, His-tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 709
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 709
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 709
    Illegal BglII site found at 491
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 709
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 709
    Illegal AgeI site found at 768
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As per above, we codon optimised for E. coli


Source

This part is a gene encoded by Acholeplasma Laidlawii. We reverse translated the protein from UniProt, using the E. coli codon optimisation tool provided by ThermoFisher's GeneArtStrings.

References